Abstracts Division 2
31. Gut microbiota perturbations precede the onset of Post-Infectious IBS in intercontinental travelers
Jiyang Chan1, Gianluca Galazzo1, 2 (shared first author), Markia Ward2, Maris Arcilla3, Perry van Genderen4 and John Penders1, 2 on behalf of the COMBAT-consortium
1 School of Nutrition and Translational Research in Metabolism (NUTRIM), Department of Medical
Microbiology, Maastricht University Medical Centre, Maastricht, the Netherlands
2 School for Public Health and Primary Care (Caphri), Department of Medical Microbiology, Maastricht University Medical Centre, Maastricht, the Netherlands
3 Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre, Rotterdam, Netherlands
4 Institute for Tropical Diseases, Havenziekenhuis, Rotterdam, Netherlands
Background
A proportion of Irritable Bowel Syndrome (IBS) patients report the onset of symptoms after infectious
gastroenteritis (IG), denoted as post-infectious IBS (PI-IBS). To what extent
microbiota alterations precede or are a consequence of the infectious episode
remains undescribed. We prospectively characterized the faecal microbiota
diversity, community structure and dynamics in Dutch intercontinental
travellers with and without PI-IBS development after travellers’ diarrhoea.
Methods
We conducted a nested case-control study within a longitudinal cohort among travellers. Cases were
defined as healthy travellers (without gastrointestinal symptoms at baseline)
who experienced diarrhoea during the index travel and met the ROME III criteria
for IBS at 6-12 months after travel return. For each case, we matched one
control based on age, gender and travel destination. Faecal samples collected
pre-travel, immediately post-travel and 1-month post-travel were profiled by
16S rRNA gene amplicon sequencing to examine the microbial diversity,
composition and community structure.
Results
53 subjects met our case definition for PI-IBS and were matched to 53 control subjects. Microbial
richness prior to the onset of PI-IBS was significantly lower in future cases
compared to controls (p=0.0028). The microbial diversity (Shannon-index)
was also lower in PI-IBS cases before and immediately after travel (p=0.0338
and p=0.0105 respectively). In addition, the microbial community
structure of PI-IBS cases was significantly different as compared to healthy
controls pre-travel, immediately after travel and one-month post-travel
(PERMANOVA p< 0.05 for all time-points). Longitudinal differential abundance
testing indicated increased levels of Bacteroides and Streptococcus
in PI-IBS cases compared to a higher abundance of Ruminococcus and Butyricoccus
among healthy controls.
Conclusions
In a longitudinal study of faecal microbiota of intercontinental travellers from pre-travel through
post-travel, we identified an altered microbiota profile preceding the onset of
PI-IBS. These results strengthen the evidence for a causal role of the
microbiota in the pathophysiology of IBS.
NUTRIM | School of Nutrition and Translational Research in Metabolism
NUTRIM aims to contribute to health maintenance and personalised medicine by unraveling lifestyle and disease-induced derangements in metabolism and by developing targeted nutritional, exercise and drug interventions. This is facilitated by a state of the art research infrastructure and close interaction between scientists, clinicians, master and PhD students.
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