D1 | Abstract 11

Annual NUTRIM Symposium 18 November 2020


Effects of beetroot powder with or without L-arginine on postprandial vascular endothelial function

Ellen T.H.C. Smeets1, Ronald P. Mensink1, Joris Hoeks1, Johan de Vogel-Van den Bosch2, Robert J. J. Hageman2 and Peter J. Joris1

1 Department of Nutrition and Movement Sciences, NUTRIM School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO Box 616, 6200 MD, Maastricht, the Netherlands.
2 Danone Nutricia Research, Utrecht, the Netherlands.
Background and aim:
Previous studies with high-dose nitrate or L-arginine supplementation have shown to improve postprandial vascular endothelial function, via their effects on nitric oxide bioavailability. Nutritional supplements containing lower and more feasible amounts of both nitrate and L-arginine might have more potential in improving postprandial endothelial function. Therefore, the aim of the present study was to compare effects of beetroot powder with or without L-arginine on postprandial endothelial function, as assessed by brachial artery flow-mediated vasodilation (FMD), after consumption of a high-fat mixed-meal in apparently healthy abdominally obese men.

This randomized, double-blinded, cross-over trial consisted of five test days, each separated by a wash-out period of at least one week. Eighteen men with a waist circumference between 102.4 and 129.0 centimeters completed all five test days. On each test day, men received a high-fat blended meal (55.3 grams of fat) with (i) beetroot powder providing 200 mg nitrate, (ii) beetroot with 0.8 grams of L-arginine powder, (iii) beetroot with 1.5 grams of L-arginine powder, (iv) 3.0 grams of L-arginine powder, or (v) a control supplement. Brachial artery FMD measurements were performed fasted and 2-hour post-consumption.

The control supplement significantly decreased postprandial FMD (P = 0.026), however, postprandial FMD values were not significantly different between meals (meal effect, P = 0.45). A trend for a more beneficial postprandial FMD value was observed for the beetroot-containing meals as compared with meals without beetroot (P = 0.074). Postprandial baseline brachial artery diameters, blood pressure, lipemia or glycemia were not significantly changed.

In apparently healthy abdominally obese men, a single dose of beetroot powder with L-arginine powder did not differently affect postprandial endothelial function. However, a trend was found for a smaller decrease in postprandial endothelial function with meals containing beetroot powder compared with meals containing no beetroot powder.

NUTRIM | School of Nutrition and Translational Research in Metabolism
NUTRIM aims to contribute to health maintenance and personalised medicine by unraveling lifestyle and disease-induced derangements in metabolism and by developing targeted nutritional, exercise and drug interventions. This is facilitated by a state of the art research infrastructure and close interaction between scientists, clinicians, master and PhD students.